Insertion junction: CLIP2.073955_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.073955
Chromosome:chromosome 9
Location:913422
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g403150 PWR9 (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein 5'UTR
Cre09.g403200 FAP198 Flagellar Associated Protein 198; (1 of 1) PTHR21281//PTHR21281:SF0 - UNCHARACTERIZED // CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTGCCCACGGGAGAGTCCCCACGGCGCGCCGCACGTTTCTTGTGCTTA
Internal bar code:AGAGAATCGTGCAGCAACAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2040
LEAP-Seq percent confirming:84.0
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:125

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGTATCTCCGCAGTGGCC
Suggested primer 2:AACAACTGCGGGTCCTGTAG