Insertion junction: CLIP2.073967_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073967
Chromosome:chromosome 11
Location:3557521
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477850 TXC1,TSN1 (1 of 1) K15979 - staphylococcal nuclease domain-containing protein 1 (SND1); Transcriptional coactivator-like protein 5'UTR
Cre11.g477900 protein of unknown function 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCAAATCCTCTGAAGGCGGAGAACGCACCAAAGGCCCCGCATAGTC
Internal bar code:TGTCTTCGTCAAACTCAGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1397
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAGCTCGATGAGATGCAC
Suggested primer 2:GCCGTTTCGATCTATCCCGT