| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074005 |
| Chromosome: | chromosome 3 |
| Location: | 1574886 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152350 | 5'UTR | ||
| Cre03.g800297 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCTTTCGTCTCCCGCTCCAAGAATAATGAAACTCCCCATCTTGCCC |
| Internal bar code: | CTGCCTCGGCGATCGCTGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTCAAGCTCCTCTGT |
| Suggested primer 2: | CTTCCACACTGACGACACCA |