Insertion junction: CLIP2.074005_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074005
Chromosome:chromosome 7
Location:5923618
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g354150 MFT22,ARSJ1 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCAACGAGGGGTGAACCGCTGGGGCGGCTTAGGGGCCTGTTGCTTGG
Internal bar code:TAGTAATCGGCACTTATAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AGATGTCTGTGGTCTGCAGC
Suggested primer 2:AGGCATCGGCTACTTCATCG