| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074033 |
| Chromosome: | chromosome 12 |
| Location: | 1580860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487250 | (1 of 1) PF06454 - Protein of unknown function (DUF1084) (DUF1084) | 3'UTR | |
| Cre12.g801367 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCACCCCATGTGCTTCAGGGTATCTAAGACAACAAGAAAGGGCCCTG |
| Internal bar code: | GCGTGACATCGCTTGGATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3770 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGCATAAGTAGGAGGAC |
| Suggested primer 2: | ACCAGGAAGAAAGAGGCGTG |