| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.074041 |
| Chromosome: | chromosome 13 |
| Location: | 4134757 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591200 | HTB38,HTB22 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCATGGTTGCGATAAGGAATGTGTTGGAGTAGGGTTGTGGGGGGCC |
| Internal bar code: | GGGCATGTGCGTTAGGGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCTTGCCAGAGGTCTT |
| Suggested primer 2: | GCTTTAGCCCGAGGTGAACT |