Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.074056 |
Chromosome: | chromosome 12 |
Location: | 2212746 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g508900 | MAPK6 | (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3); Mitogen-Activated Protein Kinase 6 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGGAGAAACCTCAGCTGGTCAGCTTGGGGGCCGGAGTAACATCTTGC |
Internal bar code: | GGGTCAGCGCGTGAGAGAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2621 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCAGCACTTGTGTTGAGTG |
Suggested primer 2: | ACCAATGTCTCCGCAATCGT |