| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074058 |
| Chromosome: | chromosome 15 |
| Location: | 744682 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801691 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTACATTAGAGCGTTATGTCTCACTTTCCTTACTTGAGTCAGTCTAG |
| Internal bar code: | AGTGGGTTCAGCAGGCGATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3899 |
| LEAP-Seq percent confirming: | 75.3247 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGAAACTCCAC |
| Suggested primer 2: | CTCCACCATTCCCACTTCCC |