| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074070 |
| Chromosome: | chromosome 4 |
| Location: | 1402247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214351 | Predicted protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCAGCCCTTCCCAACCCCGAGTGTGGGCCGTGCCTGACACTCCCGCC |
| Internal bar code: | GACAAGGCAATAAGCCAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1326 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTAGTCATCCTCGCCG |
| Suggested primer 2: | GTGTTATGTCATGCACGCCC |