| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074092 |
| Chromosome: | chromosome 16 |
| Location: | 4405597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668350 | (1 of 1) PF07889 - Protein of unknown function (DUF1664) (DUF1664) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAAACAAGCGCTCCGAATGCACGCTGCGGCGCCGGGCCCGAAGCGGT |
| Internal bar code: | GTGTCTCCAATTTATTCTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2952 |
| LEAP-Seq percent confirming: | 74.4681 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTACAGCTGTGCTTGGA |
| Suggested primer 2: | CTCGAAGGGTTCAAGCAGGT |