| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074098 |
| Chromosome: | mitogenome |
| Location: | 10727 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreMt.g802343 | ChrepMp07,nad1,801499 | NADH dehydrogenase subunit 1; (1 of 1) K03878 - NADH-ubiquinone oxidoreductase chain 1 (ND1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTTGTCTGCGTTCTGTAGCCCTTATGGTATCTTATGAGTTGAGTTT |
| Internal bar code: | TAAACCATCTAGAGTATTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2122 |
| LEAP-Seq percent confirming: | 32.6087 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGAGTCCATACAAAAGCG |
| Suggested primer 2: | AAGGGGCCACGGTTCTTATG |