Insertion junction: CLIP2.074103_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074103
Chromosome:chromosome 2
Location:6417895
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388351 PHC32 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 32 3'UTR
Cre09.g388352 (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCTGCCTCCAGTGCATGTACCCCTGTGCGCCCTCTACCACTACTCTT
Internal bar code:TGTGGCGACGTTATTGACCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:802
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTACCTAGATGGCGTC
Suggested primer 2:CGGCATTCATGGATGTGCTG