Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.074105 |
Chromosome: | chromosome 16 |
Location: | 3607209 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g672041 | (1 of 1) 2.1.1.228 - tRNA (guanine(37)-N(1))-methyltransferase / tRNA-(N(1)G37) methyltransferase | outside_mRNA | |
Cre16.g672397 | DIV174 | (1 of 3) K17499 - protein phosphatase 1G [EC:3.1.3.16] (PPM1G, PP2CG) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTAAGCGTAATTGTTGGCTTCCAACTGTTTGTTGCTATTGTCGCAG |
Internal bar code: | CGGGAAGGGGTTCGTGCACCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1023 |
LEAP-Seq percent confirming: | 90.4762 |
LEAP-Seq n confirming: | 19 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCATACCAACAGCGCCATT |
Suggested primer 2: | TCCATCGAACACGCCAAAGA |