| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074112 |
| Chromosome: | chromosome 12 |
| Location: | 8271362 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551503 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATACGATGTATGGATGAATGCACTTGTCAATACGCCATCGCCGGTGG |
| Internal bar code: | TGGGGGTCTTCTGTATTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGAGGGGAGGAGGAGT |
| Suggested primer 2: | CGCGCTGTGATTTGGTATGG |