| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074127 |
| Chromosome: | chromosome 10 |
| Location: | 206083 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419100 | Hypothetical protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGGCAGCGGAGGGAACGCAGCAGCACGAGGACCCCCCTGCACCACTA |
| Internal bar code: | GCCGTACGTGAAAGTAATAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2316 |
| LEAP-Seq percent confirming: | 95.9016 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 122 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCAGGGTCCATTGCCA |
| Suggested primer 2: | CGTTAGAATGCGTTGCGAGG |