| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074127 |
| Chromosome: | chromosome 10 |
| Location: | 206143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419100 | Hypothetical protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAGCTCACCGACAGGTCCTGCGGCGTGAGGATGATGTACTGGAAATC |
| Internal bar code: | TTAACGTTATAAAGGCGGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6574 |
| LEAP-Seq percent confirming: | 98.1707 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 164 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAGAATGCGTTGCGAGG |
| Suggested primer 2: | GAAGTCAGGGTCCATTGCCA |