| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.074141 |
| Chromosome: | chromosome 16 |
| Location: | 3052095 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664250 | OASTL2,ASL2 | O-acetylserine (Thiol)-lyase/cysteine synthase 2; (1 of 1) 4.2.1.22 - Cystathionine beta-synthase / Serine sulfhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCATGCGCGCTACACCACGCTACACCTACATAGTGCGATCCGTAGCG |
| Internal bar code: | TTTTGGATGCCAATCTACGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 18.75 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCACCACAAGCGGTAAG |
| Suggested primer 2: | TATCGCACGTTGACACCACA |