| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074148 |
| Chromosome: | chromosome 1 |
| Location: | 4880135 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034000 | IPB1 | Importin beta; (1 of 1) K14293 - importin subunit beta-1 (KPNB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCGCCTGCTCCGCGTGTGCAATGGACTTCCCCCTACTCCCTCATCGG |
| Internal bar code: | TAGACCTCCAACTGAAGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 30.9524 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTCGGAAGAGCTCGAGG |
| Suggested primer 2: | GTGCGCATTACATTCCGTCC |