| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074150 |
| Chromosome: | chromosome 3 |
| Location: | 6101182 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTTGGGTTGCATCACGTACAAGCTTGAACTAGAAACCACTGACTGCG |
| Internal bar code: | TATAACTAGTGGAAATGAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1267 |
| LEAP-Seq percent confirming: | 27.7778 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCCCTGGTCAGAATTCA |
| Suggested primer 2: | GCAGGGGTGGAGATAGATGC |