Insertion junction: CLIP2.074186_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.074186
Chromosome:chromosome 8
Location:3744525
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g380650 GT90-13,GT90F13 GT90 family protein 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGTACACACACATTTCTGCTCTCTCTCACAGACTCACACACACACG
Internal bar code:CAGGCGAGGTGTTTAAAAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAGTCAACAACCTTGCG
Suggested primer 2:CCTTGCAGCAGACACAGGTA