| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074222 |
| Chromosome: | chromosome 6 |
| Location: | 7266866 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298950 | (1 of 2) PF10343 - Potential Queuosine, Q, salvage protein family (Q_salvage) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCAACCCGGGTTCACTCGGGGCGTGCCCATACACGCCGCACCGTCCA |
| Internal bar code: | CTGACAATCGGGTTTGGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1375 |
| LEAP-Seq percent confirming: | 14.8148 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGGCCAAAGTGTGCTG |
| Suggested primer 2: | TATGGGAGAGTCACCGAGCA |