| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074228 |
| Chromosome: | chromosome 16 |
| Location: | 2788306 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662350 | TSPS1,TPS2 | (1 of 3) 2.4.1.15 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase; Trehalose-6-phosphate synthase, class I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGGCCAAAACCGCAAATGTTCACCTAGGGTGCCCCGGCCCCCTGACG |
| Internal bar code: | GGTTTAAGTTTAGGGAGCTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGTTCCATGCCAGGCTG |
| Suggested primer 2: | CAGCTCACCAAACAAGCACC |