| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074235 |
| Chromosome: | chromosome 1 |
| Location: | 2888316 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017951 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCGCAAAAACACGTGTTGTTTCAGTATCCGGGACCTACGGGCGGCG |
| Internal bar code: | AAGATTGCGCCCGCGCCGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3574 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTACTACCGCAGAGCTGA |
| Suggested primer 2: | GTCATAGAGCTTGCGTCCGA |