| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074237 |
| Chromosome: | chromosome 16 |
| Location: | 5578068 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681550 | CSV13 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCCCCGTCGGTCAAGCCGGATGCACGGCAGCGTTCATCTTATGCTG |
| Internal bar code: | ATTTACTGGAGAACGTGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4024 |
| LEAP-Seq percent confirming: | 93.7931 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 145 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTACACCTTCAGAACCC |
| Suggested primer 2: | GTTAGCGGCATTCGACAACC |