| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074241 |
| Chromosome: | chromosome 3 |
| Location: | 1529588 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152000 | ABA3,MCS1 | (1 of 2) K15631 - molybdenum cofactor sulfurtransferase (ABA3); Molybdenum cofactor sulfurase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGTGTAGGGCCATACACTAACCAGGCCGCTTTGAAGGGTGGGTACG |
| Internal bar code: | AACTGTTTCGATCTGGCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1328 |
| LEAP-Seq percent confirming: | 35.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAATATTGCATGCAGGC |
| Suggested primer 2: | AACTTTCGTCGAGCAGCTCA |