Insertion junction: CLIP2.074244_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.074244
Chromosome:plastome
Location:10376
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802266 2716978,psbK,ChreCp004 (1 of 1) K02712 - photosystem II PsbK protein (psbK); photosystem II K protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGCTAAAAACATACTCTTTACGGGTACGCGAATGTTAGGTAAATTTTC
Internal bar code:CGCGTACCAGTCCTGGTCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCTTTCAGGCAACCCAA
Suggested primer 2:CATAGGGTCGCAGGTTCGAA