| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074255 |
| Chromosome: | chromosome 13 |
| Location: | 2687778 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581500 | OPR41 | OctotricoPeptide Repeat protein 41 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGCGGGTCCAGCAGAGCTCCAGTCCAGTCGCAGAACCACGCCGGCA |
| Internal bar code: | ATGTACAATAAGTCCGTCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGACAACGTAGCCACCA |
| Suggested primer 2: | GCTGCCAGTCTTCTTGTTGC |