| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074265 |
| Chromosome: | chromosome 17 |
| Location: | 3729088 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726200 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAACAGGGCCCGGCCTCCGTATGTGGTCTGTTCCGCGCCCAACGCTAC |
| Internal bar code: | ACATCGATCATTGAGTGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5774 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACCGCATCGCTATGAC |
| Suggested primer 2: | TGGAACTGTAACCCGTTCGG |