Insertion junction: CLIP2.074269_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.074269
Chromosome:chromosome 14
Location:70642
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g608200 (1 of 1) PF12872//PF13020 - OST-HTH/LOTUS domain (OST-HTH) // Domain of unknown function (DUF3883) (DUF3883) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCTGAGGGAGGAGCAGTGGGGCGCGCTGAGGGGCGCCGTGGCGCGGC
Internal bar code:GTACTTTTTATCGGTGGAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:298
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTCTCATCCCGTTTTCGT
Suggested primer 2:AATCACCCTCGATCTCCCCA