| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074270 |
| Chromosome: | chromosome 17 |
| Location: | 6789895 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802150 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAGTCACTGTGGAGTCATTTAACTCCCTCGTAATGCTGGAAAGTTT |
| Internal bar code: | TGTACTAAAGGTTTTACGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCAGGGTGTTCGTTCT |
| Suggested primer 2: | TCAACTGGTTCAGGTACGCC |