| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074276 |
| Chromosome: | chromosome 2 |
| Location: | 1435998 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083550 | (1 of 1) PF01494//PF05834 - FAD binding domain (FAD_binding_3) // Lycopene cyclase protein (Lycopene_cycl) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACACGCTTACCCACGTACACATATACACACATTTTACCTTCGTGCTT |
| Internal bar code: | AGTCACGGGCTAATAATGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATGACATTGGATGGCTG |
| Suggested primer 2: | CCAGAACTTCCGGTGCTCAT |