| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074280 |
| Chromosome: | chromosome 13 |
| Location: | 539755 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565321 | (1 of 2) 4.3.2.2 - Adenylosuccinate lyase / Succino AMP-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAATGCCGTCCACGAAGGTCATCATCGACTCCTGTGTTGGTGTGTAGT |
| Internal bar code: | TGCTTGTCCTAGCTTGCCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGGGCATCTCCAAGCTG |
| Suggested primer 2: | TTTTGTTCGCCGTCAACGTC |