| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074281 |
| Chromosome: | chromosome 2 |
| Location: | 3947252 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102250 | RPS3 | Cytosolic 80S ribosomal protein S3; (1 of 1) K02985 - small subunit ribosomal protein S3e (RP-S3e, RPS3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGAGAGAAAGAGCGCGGTGGGGCCCAGTTGCTAAATACTCTGCATGG |
| Internal bar code: | GGCCACCGGTCTTTTTCGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3461 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCATCGGACTAGCACG |
| Suggested primer 2: | TTGAAGCGCTTCTGCACAAC |