Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.074284 |
Chromosome: | chromosome 7 |
Location: | 3598453 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g336850 | (1 of 3) PF01661 - Macro domain (Macro) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCCTCGGCGCCTCGGCCTCGGCGCCTCGGTTACCAAGTGCCAACTA |
Internal bar code: | GCGGATACCGTTGGTGCGGTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4160 |
LEAP-Seq percent confirming: | 89.4737 |
LEAP-Seq n confirming: | 17 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTACGGGTTCCAGTGGCTC |
Suggested primer 2: | CCTCTGCAATACGTGGGGTT |