| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074288 |
| Chromosome: | chromosome 3 |
| Location: | 9160925 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200095 | (1 of 2) K11594 - ATP-dependent RNA helicase DDX3X (DDX3X, bel) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGCCAAGTCAAAAACAGCCTGTGCAGGAGGTGAACAGGGCAATGCAC |
| Internal bar code: | ATGGTGTAGTGCCGGCCTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2970 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTAAGTGGCGACGTGCT |
| Suggested primer 2: | AATCCTACCTGCACAGTGGC |