Insertion junction: CLIP2.074293_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074293
Chromosome:plastome
Location:136472
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802320 chlN,2716986,ChreCp056 light-independent protochlorophyllide reductase subunit N; (1 of 1) K04038 - light-independent protochlorophyllide reductase subunit N (chlN) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGATAATTTATTAGAAATTTCTTTAGCACGTTTTTTAACACGTTGTG
Internal bar code:GCACAGGCTATTCCTATGTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCAGACCACACAAACCAT
Suggested primer 2:CCCATTGTTGTAGCACGTGC