| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074294 |
| Chromosome: | chromosome 9 |
| Location: | 829365 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403750 | HAD6 | Halo-acid dehalogenase-like hydrolase; (1 of 1) PTHR12725:SF55 - F14L17.7 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTTCCGGGATGACTAAAAGTCATAGCTGCAGATACACGGACGGTCA |
| Internal bar code: | TCGAACTAGGATCCAAGACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 38.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACACGTGAGGACTTCTC |
| Suggested primer 2: | GCCCACCAGCAACTGTATCT |