| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074298 |
| Chromosome: | chromosome 4 |
| Location: | 3283171 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226811 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGCAGTCAGTCCTGGCAGCAGCTCCGCTGAAGCAGCGTCAAGCAAC |
| Internal bar code: | GCGCCATATAACAAAGCAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2389 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTCCCTTCCATCGGCTA |
| Suggested primer 2: | ACTGCATGTAAGTTGGCGGA |