Insertion junction: CLIP2.074301_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.074301
Chromosome:chromosome 12
Location:7060313
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g560250 (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCAAACCGACCTGTTGCCGTGCACCCACCCACGCGCCCTGTAAAGTC
Internal bar code:GATCCACTTCGATGTGCTGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1113
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTCTTGGATCCTTGGTGG
Suggested primer 2:CAAACTGCGAGTGTTGTGGG