| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074304 |
| Chromosome: | chromosome 3 |
| Location: | 767694 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146087 | (1 of 3) 1.14.11.33 - DNA oxidative demethylase / Alkylated DNA repair protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCATAAAACTGGTTGTGTCCCATACGAGTGTCCTGACTTGCTGCGTG |
| Internal bar code: | GTTAACATTTGGTCTGCCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1525 |
| LEAP-Seq percent confirming: | 76.4706 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAAGCGTGACTGGGTCCA |
| Suggested primer 2: | GTCGCTGGTGTTCAAGGAGA |