| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.074309 |
| Chromosome: | chromosome 17 |
| Location: | 775202 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701300 | (1 of 1) K17491 - protein phosphatase 4 regulatory subunit 3 (SMEK, PPP4R3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGTGTGCGTTGGCCCATTGGCCACCGCTTCGTCCCCACCCGCCGTC |
| Internal bar code: | TCGATGATGATAAGGGAGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCATCACCATGCAGCAGC |
| Suggested primer 2: | TAACGGTCAACACGTCAGGG |