Insertion junction: CLIP2.074316_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.074316
Chromosome:chromosome 3
Location:2132102
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g156750 CCT5 T-complex protein 1, epsilon subunit; (1 of 1) K09497 - T-complex protein 1 subunit epsilon (CCT5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCATCTGCGGGTGGCTGAACTCCTTGTCGATGACAATGCCCTCCACC
Internal bar code:GCGTAAGACCATTATTCTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:338
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGATTGTATGTTGGAGCGCG
Suggested primer 2:GTAGTGCTACCCTAACCCGC