| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074356 |
| Chromosome: | chromosome 8 |
| Location: | 3395170 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377950 | (1 of 3) PF02622 - Uncharacterized ACR, COG1678 (DUF179) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGTGCCACTACCCGAGTGATTGCCTTTTTGTGTGTATATATACCGAC |
| Internal bar code: | GGCCGTGAGGAGGTTGGCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2192 |
| LEAP-Seq percent confirming: | 95.3488 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACAAAAGGTGCGGTGAC |
| Suggested primer 2: | AGCCACCTCCATCAGATCCT |