Insertion junction: CLIP2.074358_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074358
Chromosome:chromosome 17
Location:2720544
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802055 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATAGTTAGTGGGTGGGCTTTGGGATGCGTGTTAGGACATTAGATAGC
Internal bar code:GCTCCGACTAGTGCAAGGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3649
LEAP-Seq percent confirming:92.0635
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GGATCTAGGACCTGAAGCGC
Suggested primer 2:TTTCTGGGTACGGTTTCGGG