| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074360 |
| Chromosome: | chromosome 9 |
| Location: | 3543720 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396512 | OPR12 | OctotricoPeptide Repeat protein 12 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTGGCGGCGAACCACCTTCACGGGGAACGCACAACGGCACACAGACG |
| Internal bar code: | GTACGGTGCAATTCGCTTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3516 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGGATGGGCTGCTATG |
| Suggested primer 2: | CGGACTGTCCCGTCTTTGAA |