| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074362 |
| Chromosome: | chromosome 1 |
| Location: | 5700379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040801 | (1 of 3) PTHR18937//PTHR18937:SF254 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTATGGGTGCGCCGCGCCCCCTGCGGGGGCCGGGGCACGCCAACCA |
| Internal bar code: | TCGTGTAACATAGATATCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4318 |
| LEAP-Seq percent confirming: | 98.9474 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGACAGTCATGGAGCC |
| Suggested primer 2: | CCGCGAGATACAGACTCCAC |