| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074363 |
| Chromosome: | chromosome 2 |
| Location: | 5976977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118300 | RHE,HEL12 | (1 of 1) PTHR24031:SF330 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 7; DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGGTTTGCGCTTGCGCTGCGGATGTGCGGGCAGGTGGGGGGTTGCC |
| Internal bar code: | ATATTGGTAAGTGTACTAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1218 |
| LEAP-Seq percent confirming: | 89.7436 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGGTAGAGGTAGGGCAG |
| Suggested primer 2: | TTGCTGTATGATGGCCCCAG |