Insertion junction: CLIP2.074367_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.074367
Chromosome:chromosome 8
Location:3053018
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre08.g375350 SDR12 (1 of 2) K19329 - WW domain-containing oxidoreductase (WWOX); Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACCACCACCACCACCATGCATGCACGTTTTGCGGTTCTCATTC
Internal bar code:TGTAGTATCGTCATAGAACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:974
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCCAAACCAACACCAC
Suggested primer 2:CGAAGGAACATGTTAGCGCG