| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074371 |
| Chromosome: | chromosome 12 |
| Location: | 7659498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555350 | RER1 | (1 of 1) PTHR10743 - PROTEIN RER1; ER retention protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCAGCAGTGGAGGGGTGGACTGAGGTACGGTTTGTTCGGGGCGCGG |
| Internal bar code: | TTACTGGATTAGTAGGCCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4919 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGATGACTTTGACCGCC |
| Suggested primer 2: | ACTCGTTTTCCTACGCGGTT |