Insertion junction: CLIP2.074374_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.074374
Chromosome:chromosome 13
Location:3850081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGCTGAGACATCGCGCCGCTTGAGCGGCACCGGGATCGGCTTCTCAC
Internal bar code:AACTTGGGGCGGCCTGCAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:8.62069
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAACGTTCTCGCACACGG
Suggested primer 2:GGAGAGGACGTAGGTCACCT